• Measurement of LOX inhibition by compounds
• Positive control in immunodiagnostics
• Positive control as LOX enzyme in comparison to LOXL

enzymes

• Identification/characterization of substrates of LOX

Lysyl oxidase (LOX, Acc.# NM_173932.4, similar to Human Acc.#
NM_001178102, EC 1.4.3.13) is a copper-containing 32 kDa protein found in structural tissues, where it functions to promote
crosslinking of structural proteins, resulting in higher tensile
strength of the biopolymer. Although concentrations are up to 500
µg/ml in some tissues (e.g., tendon), the yields are very low in the
purified form, usually 1-2 mg per Kg of raw material (Kagan and
Cai, 1995). LOX is supplied as a white lyophilized powder.

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >80% pure.