• Study in vitro regulation of N-Ras cell signaling mechanisms
• Serves as a substrate for N-Ras GEF assays and drug screens

N-Ras is a small GTP-binding protein in the Ras family that plays a key role in regulating cell growth, differentiation, and survival through signal transduction pathways. Mutations in the N-RAS gene are implicated in various cancers, where they can lead to uncontrolled cell proliferation.

Wild-type human N-Ras protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: approximate mwt 23-25 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 50 mM Tris pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran.

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of N-Ras can be determined from the ability of the RasGRF1 exchange domain (Ras-GRF1-ExD) to catalyze the exchange of GDP for GTP on N-Ras.