• Rapid/transient activation of Rac/Cdc42 proteins in live cells
• Positive control for Rac/Cdc42 activation in live cells
• Study the mechanisms underlying Rac/Cdc42-mediated events

Part of the G-Switch™ line, CN02 (epidermal growth factor: EGF) acts through a tyrosine kinase receptor (EGFR) to rapidly stimulate actin reorganization through activation of Rac and/or Cdc42 small G-proteins. EGF is a valuable tool to study Rac/Cdc42 signaling pathways. It should be noted that EGF activates several other critical signal transduction pathways, including Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT, and data should be interpreted accordingly.

• Molecular weight: 6 kDa
• CAS number: 62229-50-9

Preparation & Use:

• Supplied as a lyophilized powder
• Reconstitute each tube in 100 µl sterile phosphate-buffered saline pH 7.0 (PBS) to give 10 units of reagent (0.1 unit/µl)
• Recommended working concentration: 1 unit/ml
• Each tube is sufficient for 10 ml of working-strength solution

≥98% by HPLC

Biological activity of CN02 is demonstrated by its ability to activate Rac1 and Cdc42 in live Swiss 3T3 cells. Treatment at 1 unit/ml produces optimal activation, approximately twofold, within 1-2 minutes as measured by a Rac1BK128 and Cdc42 BK127 G-LISA assays. Responses to CN02 will vary depending on the cell line used.

• Activation timeline: typically 0.5-5 minutes to activation peak
• Activation duration: typically 1-5 minutes
• Typical activation vs untreated: 2-3 fold