• Rapid/transient activation of Rho proteins in live cells
• Positive control for Rho activation in live cells
• Study upstream regulators of Rho
• Study the mechanisms underlying Rho-mediated events

Part of the G-Switch™ line, CN01 (calpeptin) activates RhoA, RhoB, and RhoC in a variety of cell types through indirect activation. The mechanism involves inhibition of Shp-2 phosphatase, which results in constitutive activation of Rho GEFs.

In addition to its Rho-related effects, CN01 inhibits calpain-1 through a separate, unrelated mechanism.

• Peptide sequence: Z-Leu-Nle-CHO
• Molecular weight: 362.5
• CAS number: 117591-20-5

Preparation & Use:

• Supplied as a lyophilized powder
• Reconstitute each tube in 50 µl DMSO to give 10 units of reagent
• Recommended working concentration: 1 unit/ml
• Each tube is sufficient for 10 ml of working-strength solution

≥95% by HPLC

Biological activity of CN01 is demonstrated by its ability to activate RhoA in live Swiss 3T3 cells. Treatment at 1 unit/ml produces optimal activation, approximately twofold, within 10-20 minutes as measured by a RhoA G-LISA assay BK124. Rho activation by CN01 is typically more sustained than growth factor activations, which typically peak in 1-2 minutes and then rapidly decline. Responses to CN01 may vary depending on the cell line used.

• Activation timeline: typically 10-20 minutes to activation peak
• Activation duration: typically 30-40 minutes
• Typical activation vs untreated: 2-3 fold