Conjugation of SiR-NHS dye to proteins or peptides
Conjugation of SiR-NHS dye to oligonucleotides or small molecules bearing a primary or secondary amine group
Material
SiR-NHS is an amine reactive dye based on silicon rhodamine (SiR) (Ab: 652, Em: 674), designed for efficient conjugation to proteins, peptides or other amine-containing molecules.
Key features
Ab: 652 nm, Em: 674 nm – far-red spectrum
Emission in far-red minimizes phototoxicity and sample autofluorescence
High extinction coefficient
High photostability
Compatible with widefield, confocal, STED and SIM microscopy
