• Monitor tubulin polymerization in vitro via kinetic optical density increase
• Robust assay for characterization of anti-cancer compounds and MAPs targeting microtubule dynamics
• Characterize microtubule-binding proteins (MAPs)
• Determinations IC50 & EC50 for anti-tubulin ligands

Kit content (sufficient for 24-30 assays)

• Tubulin protein (99% pure)
• Tubulin glycerol buffer
• Guanosine triphosphate (GTP)
• Paclitaxel (polymerization enhancer)
• General tubulin buffer
• DMSO
• Half area 96 well plate
• Detailed protocol

Equipment & materials required

• Protein or compound of interest
• Temperature-controlled spectrophotometer capable of reading a 96-well plate at 340 nm in kinetic mode

This kinetic assay measures tubulin polymerization dynamics by measuring light scattering (absorbance) as tubulin polymerizes into microtubules. It is designed to monitor the real-time kinetics of microtubule assembly and disassembly, which is critical for understanding cellular functions and screening pharmacological agents.

Key features

• Absorbance-based readout at 340 nm
• 300 µg tubulin per assay/10% glycerol, 24-30 assays
• 100 µl volume per assay
• Coefficient of variation (cv): 13%
• Paclitaxel EC50: 0.20 µM under standard conditions
• Vinblastine IC50: 0.88 µM under standard conditions