• Control beads for UBA01B-beads UBA01B-beads ensuring specificity of ubiquitin signal in affinity enrichment
• Allow accurate interpretation of ubiquitin enrichment data

When performing ubiquitinated protein enrichment, it is important to include a control bead that is as similar to the enrichment beads as possible, but cannot bind ubiquitinated proteins. The CUB02B-beads contain two point mutations in the ubiquitin affinity binding domains of the ubiquitin enrichment beads Cat # UBA01B-beads that result in loss of Ub binding and offer an excellent control bead for Ub enrichment.

CUB02B-beads comprise non-Ub binding TUBE protein (due to point mutations) that has been co-valently attached to agarose beads. The point mutation

Key characteristics

• Same mg/ml protein content as UBA01B-beads
• Very similar amino acid composition to UBA01B-beads
• Sufficient reagent for 10 IP assays (1 mg lysate per IP)
• Consistent lot-to-lot performance
• Supplied as a lyophilized powder
• Buffer composition after rehydration: 80 mM PIPES pH 7.2, 4% sucrose, and 4% polyethylene glycol