• Study in vitro regulation of Rac2 cell signaling mechanisms
• Serves as a substrate for Rac2 GEF assays and drug screens
• Serves as a substrate for Rac2 GAP assays and drug screens

Rac2 is a small GTP-binding protein in the Rho family that regulates actin cytoskeleton reorganization, cell migration, and reactive oxygen species production in immune cells. It plays a critical role in innate immunity, hematopoietic cell signaling, and inflammatory responses.

Wild-type human Rac2 protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: approximate mwt 22-25 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 50 mM Tris pH 7.5, 0.5 mM MgCl2, 50 mM NaCl, 0.5% (w/v) sucrose, and 0.1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of RC02 is evaluated by measuring its GTPase activity—its ability to hydrolyze GTP. Strict quality control ensures that His-Rac1 exhibits at least a threefold increase in GTP hydrolysis rate in the presence of the Rac GAP, p50 RhoGAP.