• Study in vitro regulation of Rac1 cell signaling mechanisms
• Serves as a substrate for Rac1 GEF assays and drug screens
• Serves as a substrate for Rac1 GAP assays and drug screens

Rac proteins are small GTP-binding proteins that regulate actin-based structures such as lamellipodia, membrane ruffles, and dorsal ruffles, enabling dynamic remodeling of the cytoskeleton. They are essential for processes including cell migration, adhesion, and growth factor–mediated signaling.

Wild-type human Rac1 protein is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag for purification
• Molecular weight: approximate mwt 45 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 50 mM Tris pH 7.5, 100 mM NaCl, 1.0 mM MgCl2, 5.0% sucrose, and 1.0% dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of RCG01 is evaluated by measuring its GTPase activity—its ability to hydrolyze GTP. Strict quality control ensures that GST-Rac1 exhibits at least a threefold increase in GTP hydrolysis rate in the presence of the Rac GAP, p50 Rho GAP.