• Study in vitro regulation of RhoC cell signaling mechanisms
• Serves as a substrate for RhoC GEF assays and drug screens
• Serves as a substrate for RhoC GAP assays and drug screens

RhoC is a small GTPase in the Rho family that plays a pivotal role in regulating actin cytoskeleton dynamics, particularly in cell motility and invasion. It promotes actin filament assembly through downstream effectors such as ROCK and mDia, driving stress fiber formation, focal adhesion turnover, and cytoskeletal remodeling.

Wild-type human RhoC protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: approximate mwt 24-28 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 20 mM Tris, pH 7.6, 0.5 mM MgCl2, 0.5% (w/v) sucrose, and 0.1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of RH03 is evaluated by measuring its GTPase activity—its ability to hydrolyze GTP. Strict quality control ensures that His-RhoC exhibits ≥2 fold increase in GTP hydrolysis rate in the presence of 40 mM EDTA.