• Study in vitro regulation of H-Ras cell signaling mechanisms
• Serves as a substrate for H-Ras GEF assays and drug screens

H-Ras is a small GTP-binding protein that plays a critical role in cell signaling, regulating pathways involved in cell growth, differentiation, and survival. Mutations in H-Ras can lead to uncontrolled cell proliferation and are implicated in various cancers and developmental disorders.

Wild-type human H-Ras protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: approximate mwt 25-28 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 2 mM Tris, pH 7.6, 0.5 mM MgCl2, 0.5% (w/v) sucrose, and 0.1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥80% pure.

The biological activity of H-Ras is measured by its ability to exchange GDP and GTP. In the assay, EDTA induces a conformational change in His-Ras that mimics GEF activity—using the Rho GEF Exchange Assay Kit Cat. # BK100 quality control confirms that 40 mM EDTA doubles the exchange rate (Vmax) of His-Ras.