As part of the Signal-Seeker™ product line, acetyl-lysine control beads (CIG02) have been developed as highly specific negative control beads for Cytoskeleton's acetyl-lysine antibody-based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. The IgG antibodies are covalently attached to minimize antibody heavy and light chain leaching, which makes interpretation of results easier. Additionally, these control beads are effective for pre-clearing lysates without risk of contamination with IgG antibody. IgG control beads (CIG02) are included in our comprehensive Signal-Seeker™ Acetyl-Lysine Detection Kits and utilizing these kits are recommend for first time acetyl-lysine detectiion.
Validation Data: Acetyl-Lysine Control Beads White Paper
Each lot of affinity-bead is quality controlled to provide high batch to batch consistency, see COA documents.
Application 1: Utilization of acetyl-lysine control beads
Acetyl-lysine control beads are compatible with Acetyl-Lysine Affinity Beads and Signal-Seeker Acetyl-Lysine Detection Kits. Note these beads do not have cross reactivity with Acetyl-Lysine (Figure 1).
Figure 1: COS cell either untreated or treated with TSA and nicotinamide were lysed with BlastR buffer. 1mg of protein was incubated with AAC04-beads or CIG02-beads. Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using an Acetyl-lysine-HRP antibody (AAC03-HRP) was performed.
Each package contains enough IgG control beads for 10 reactions.
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Signal-Seeker™ Acetyl-Lysine Detection Kit (Cat. # BK163)
Signal-Seeker™ Acetyl-Lysine Affinity Beads (Cat.# AAC04-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
|Horita, Henrick et al.||Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteins||Journal of Visualized Experiments||2018||ISSN 1940-087X|
|Horita, Henrick et al.||Utilizing optimized tools to investigate PTM crosstalk: Identifying potential PTM crosstalk of acetylated mitochondrial proteins||Proteomes||2018||ISSN 2227-7382|
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