• Detect endogenous acetylation in your protein of interest (POI)
• Discover novel regulatory mechanisms for your POI
• Confirm acetylation data generated through the transfected POI
• Confirm acetylation data generated through proteomic approaches
• Discover novel biomarkers

Kit contents (10 - 30 assays)

• BlastR® lysis buffer
• BlastR® dilution buffer
• BlastR-2® wash buffer
• Trichostatin A (Class I & II HDAC inhibitor)
• Nicotinamide (Class III HDAC inhibitor)
• Protease inhibitor cocktail
• Acetyl lysine affinity beads (AAC02 & AAC03 beads)
• Control beads
• Anti-acetyl lysine-HRP antibody
• Precision Red™ advanced protein assay reagent
• Bead elution buffer
• DMSO
• Chemiluminescent detection reagents
• Spin column collection tubes
• BlastR® filters

Equipment & materials required

• Cell line or tissue
• Cell scrapers
• Liquid nitrogen for snap freezing lysates
• SDS-PAGE & Western blot equipment
• Primary antibody to POI
• Secondary antibody

The Signal-Seeker® acetyl-lysine detection kit uses affinity beads to enrich acetylated proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard Western blot procedures, and the modified protein of interest is detected by the end-user using their own primary antibody.

Key characteristics

• Sensitivity: Demonstrated to detect endogenous acetylation in multiple target POI
• Input requirements: 200 µg – 1 mg of lysate
• Affinity beads: Contains affinity reagents AAC02-Beads and AAC03-Beads
• Timeframe: Approximately 10 hours, typically performed over two days