• Study in vitro regulation of Cdc42 cell signaling mechanisms
• Substrate for identification of Cdc42 binding proteins

Cdc42 is a small GTP-binding protein in the Rho family that regulates cell polarity, cytoskeletal organization, and signal transduction. It is best known for controlling the formation of filopodia—thin, actin-rich projections that help cells sense their environment and guide directional movement.

The constitutively active form of the human Cdc42 protein is produced in a bacterial expression system. The protein has a glutamine to leucine substitution at amino acid 61, creating a constitutively active mutant protein that will not hydrolyze GTP.

• Protein tag: N-terminal His tag for purification
• Molecular weight: approximate mwt 22-25 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 1 mg/ml, the buffer composition is: 10 mM Tris pH 7.5, 10 mM NaCl, 0.1 mM MgCl2, 1.0% sucrose, and 0.2% dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥70% pure.

The biological activity of C6101 is measured by its ability to mimick a GTPγS loaded wild type Cdc42 in a G-LISA assay BK127.