• Fluorogenic, bright, far-red stain for DNA in live cells and tissues
• Fluorogenic, bright, far-red stain for DNA in fixed cells and tissues
• Fluorogenic, bright, far-red nuclear stain for live and fixed cells and tissues

SPY650-DNA reagent is a conjugate of SPY™ dye and the DNA minor groove binder bisbenzimide (Hoechst). It allows the labelling of DNA in live cells with high specificity and low background.

Key features

• Ab: 652 nm, Em: 674 nm – far-red spectrum
• Emission in far-red minimizes phototoxicity and sample autofluorescence
• Improved live cell permeability over SiR-DNA CY-SC007
• Bright: brightest far-red DNA stain
• Works on live cells and fixed cells or tissues
• Fluorogenic character: enhanced fluorescence when bound to DNA
• Compatible with widefield, confocal, SIM or STED imaging

The biological activity of CY-SC501 is assessed by the ability of the probe to efficiently label DNA/nuclei in live HeLa cell culture. Imaging performance has determined this probe to be less toxic and to have superior brightness, stability, and live cell permeability than the SiR-DNA probe. After an optional wash step, cell staining is visible for several hours. Omit the wash step for time-lapse imaging.