• Visualize fluorescent fibronectin incorporation, remodeling, and degradation in live-cell imaging studies of cell/ECM interactions
• FACS analysis of fibronectin-binding cells
• Cell invasion assays
• Mechanobiology and tissue engineering research

Fibronectin is purified from bovine plasma; it is a high-molecular-weight (~440kDa) glycoprotein, made up of two subunits that vary in size between 235-270 kDa (due to alternate splicing). The secreted fibronectin dimer is a soluble protein that polymerizes to higher-order fibrils in the extracellular matrix (ECM).

The protein is modified to contain covalently linked HiLyte™647 at random surface lysines.

• Ab: 640 nm Em: 670 nm – far-red spectrum
• Labeling stoichiometry: ~1-3 dyes per fibronectin dimer
• <5% free dye detectable in final product
• Supplied as a lyophilized powder

Protein purity is determined by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. FNR04 is >80% pure.

Biological activity of FNR04 is demonstrated by its ability to act as a substrate for cell invasion, as monitored through an ECM degradation assay.