• Detect changes in plasma membrane polarity
• Fluorogenic imaging of the plasma membrane

MemGlow™NR12A is a solvatchromic probe that changes its emission spectra in response to a change in membrane (solvent) polarity. The probe acts like an on/off switch, upon binding with a plasma membrane in a predominant liquid ordered (LO) phase, NR12A exhibits a 45-50 nm wavelength shift relative to the liquid disordered (Ld) phase, enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma membranes.

Key features

• Ab: 554 nm, Em: 638 nm – orange/red – spectrum
• Polarity shift: <585 nm (ordered), >585 nm (disordered)
• Compatible with super-resolution microscopy and conventional widefield and confocal microscopy

The biological activity of MG07 is assessed by the ability of the probe to efficiently label plasma membranes in live HEK293 cell culture.