As part of the Signal-Seeker™ product line, Ubiquitin affinity beads have been optimized in order to detect endogenous levels of mono- and poly-ubiqutinated proteins, which often represent <1% of the target protein. UBA01B-Beads can enrich all ubiquitinated species with a high affinity, including mono-, multi- and polyubiquitin chains (see Application 1 below). The affinity moeities have been cross-linked to beads for low leaching and cleaner detection of the protein of interest. A comprehensive Signal-Seeker™ Ubiquitination Detection Kit is also available (BK161) and is recommended for first time users.
Note: The product #s have been updated from UBA01 and CUB02 to UBA01B and CUB02B beads due to a reformulation of the bead preservation buffer. Reformulation results in an enhanced bead performance post-lyophilization. The affinity protein formulation on the beads has not changed.
Figure 1: Swiss 3T3 cells were pre-treated with MG-132, then either untreated or treated with CNF1 (CN04) for 3 hours prior to lysis with BlastR buffer. The BK161 kit was utilized to perform the IP on 300 μg of lysate per condition. Lane 1: 3 μg input untreated lysate, Lane 2: Ubiquitin Affinity beads (UBA01) plus untreated lysate, Lane 3: UBA01 plus CN04 treated lysate, Lane 4: ubiquitin control beads (CUB02) plus untreated lysate, Lane 5: CUB02 plus CN04 treated lysate. Samples were analyzed for Rac1 ubiquitination using an anti-Rac1 antibody.
Each package contains enough ubiquitin beads for 40 reactions and sufficient control beads for 5 reactions.
For more information contact: firstname.lastname@example.org
Signal-Seeker™ Ubiquitination Detection Kit (Cat. # BK161)
Signal-Seeker™ SUMOylation 2/3 Detection Kit (Cat. # BK162)
Signal-Seeker™ Phosphotyrosine Detection Kit (Cat. # BK160)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
Signal-Seeker™: PTMtrue™ Ubiquitin Antibody (Cat.# AUB01)
|Nguyen, Kha The et al.||The MARCHF6 E3 ubiquitin ligase acts as an NADPH sensor for the regulation of ferroptosis||Nature Cell Biology||2022||ISSN 1476--4679|
|Dieter, Sebastian M. et al.||Degradation of CCNK/CDK12 is a druggable vulnerability of colorectal cancer||Cell Reports||2021||ISSN 2211-1247|
|Gu, Chunming et al.||Identification of berberine as a novel drug for the treatment of multiple myeloma via targeting UHRF1||BMC Biology||2020||ISSN 1741-7007|
|Horita, Henrick et al.||Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteins||Journal of Visualized Experiments||2018||ISSN 1940-087X|
|Horita, Henrick et al.||Utilizing optimized tools to investigate PTM crosstalk: Identifying potential PTM crosstalk of acetylated mitochondrial proteins||Proteomes||2018||ISSN 2227-7382|
|Horita, Henrick et al.||A simple toolset to identify endogenous post-translational modifications for a target protein: A snapshot of the EGFR signaling pathway||Bioscience Reports||2017||ISSN 1573-4935|