• Detect endogenous acetylation changes on a protein of interest (POI) in cells or tissue extracts
• Isolate the total acetylation profile from cells or tissues via immunoprecipitation (IP) for downstream proteomic analysis
• Confirm POI acetylation data generated from mass spec proteomics

Cytoskeleton offers two Acetyl-Lysine Affinity beads: AAC02-beads and AAC03-beads. Both contain mouse anti-acetyl-lysine antibodies covalently linked to protein G beads and enrich a broad range of acetylated proteins. Used together, they provide a more comprehensive enrichment profile.

While their specificities overlap, each has unique strengths—AAC02-beads may outperform AAC03-beads for specific targets. For a new protein of interest, test both separately to determine which gives the best results (see Detailed Methods and Validated Applications for examples).

AAC03-beads are an affinity reagent comprising a high-affinity/specificity pan-acetyl-lysine antibody AAC03 covalently linked to agarose beads.

Key characteristics

• Broad enrichment of acetylated proteins
• No heavy/light chain leaching during IP
• Complimentary overlap with AAC02-beads AAC02-beads - see datasheet for details
• Sufficient reagent for 40 IP assays (1 mg lysate per IP)
• Consistent lot-to-lot performance
• Supplied as a lyophilized powder
• Buffer composition after rehydration: 200 mM PIPES, 50% glycerol, 5% sucrose, and 1% dextran

The biological activity of AAC03-beads is demonstrated by the reagent’s ability to IP a broad range of acetylated proteins from cell and tissue lysates (50 µl bead slurry:1 mg input lysate). Specificity is demonstrated by the fact that the IP acetylation profile increases by ≥10 fold in lysate treated with the deacetylase inhibitors trichostatin A (TSA) and nicotinamide.