• Fluorescent staining of fixed cells and tissues
• Preparation of fluorescent actin filaments in vitro
• Compatible with super-resolution microscopy

Acti-stain™555 fluorescent phalloidin is a high-affinity probe for F-actin, enabling precise visualization of the actin cytoskeleton in fixed cells and tissues. Conjugated to bright, photostable fluorophores, it delivers sharp, low-background staining ideal for fluorescence microscopy and quantitative imaging applications.

Key characteristics

• Ex: 550 nm, Em: 570 nm - orange-spectrum
• Specific for F-actin (Kd 20 nM)
• Low background, bright, stable signal
• Cost-effective: sufficient stock for ≥70 ml 1x reagent
• Formulation: Supplied as a lyophilized powder
• Compatible with widefield, confocal and super resolution microscopy techniques, including PAINT, SIM, STORM, and other SMLM methods

≥90% by HPLC

Biological activity of Acti-stain™555 is demonstrated by the ability of phalloidin to specifically stain actin filaments in 3T3 cells.