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The Precision Red Advanced Protein Assay Reagent is designed to optimize the speed and accuracy of protein measurement. The reagent combines the useful properties of low protein to protein variance (See Fig 1) an d a wide protein concentration for a robust assay. The assay can measure accurately protein concentrations ranging from 0.25 to 50 mg/ml and is detergent compatible. A simple one step procedure results in a red to blue color change within 1 min, which can be recognized by measuring absorbance at 600 nm.
This reagent is so reproducible with a wide range of proteins and protein concentrations that it is employed to accurately determine the concentration in all of Cytoskeleton, Inc's protein products.
The Precision Red Advanced Protein Assay makes it very easy and quick to measure prottein concentrations. A general method looks as follows:
Detergent and chemical compatibility
The Precision Red Advanced Protein Assay is a highly stable assay and it tolerates a wide range of reagents. See the Advanced Protein Assay reagent compatibility page for a full list of reagents that have been tested.
Using the Precision Red Advanced Protein Assay in cell extracts
Since the Precision Red Advanced Protein Assay is highly compatible with non-ionic detergents (see the ADV Chemical tolerance page), this reagent is ideal for measuring protein concentrations in cell and tissue extracts. Furthermore, since protein concentration can be measured after only 1 min of incubation with the reagent, it allows the user to proceed with the experiment quicker than any other protein assay, something that can be of big importance when working with labile cell extracts.
Cytoskeleton, Inc. recommends using ADV02 for all our kits and assays that involve cell or tissue lysis. In the G-LISA™ small G-protein activation kits, we have even included this reagent to ensure that the user gets the most accurate and quickest possible measurement of the protein concentration in the cell lysates generated.
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Zilberter et al., 2013. Dietary energy substrates reverse early neuronal hyperactivity in a mouse model of Alzheimer's disease. J. Neurochem.10.1111/jnc.12127.
Question 1: Can I change the dilution volume of the experimental lysate being assayed?
Answer 1: Yes, different volumes of lysate can be used to quantify protein levels. The equation to determine protein concentration in µg/ml is: OD reading x dilution factor x 100 µg/ml/cm if a small volume (1.0 ml) cuvette is used. If a 96 well plate is used, the same equation is used with this single modification: 125 µg/ml/cm is the value used rather than 100 µg/ml/cm.
Question 2: Can this assay be performed using 96 well plates?
Answer 2: Yes, the protein assay can be performed in either small volume cuvettes (1.0 ml) or a 96 well plate. If a 96 well plate is used, the equation to determine protein concentration in µg/ml is: OD reading x dilution factor x 125 µg/ml/cm.
If you have any questions concerning this product, please contact our Technical Service department at firstname.lastname@example.org