Recombinant human KIF10 motor domain purified as a GST-tagged protein; exhibits high purity and robust activity suitable for in vitro assays, including small molecule inhibitor screening.

• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF10 (CENPE, kinesin-7) inhibitors
• Counter-screen for kinesin drug candidates

Human KIF10 (CENPE, kinesin-7) is a motor protein essential for chromosome alignment during cell division, ensuring accurate segregation in mitosis. It plays a critical role in kinetochore–microtubule interactions, and its dysfunction can contribute to chromosomal instability.

The wild-type human motor domain of KIF10 (MKLP2) is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag
• Molecular weight: 71 kDa
• Motor amino acids: 1-395
• Uniprot ID: Q02224
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100, 6% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >90% pure.

Biological activity of KIF10 is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF20A exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 900 nmol of ATP produced per minute per mg of KIF10.