SiR-lysosome is based on the pepstatin A (a cathepsin D binding peptide) which has been labeled with the proprietary fluorophore silicon rhodamine (SiR). SiR is a bright and photostable rhodamine-like dye, but with advanced properties. SiR-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. It stains lysosomes in live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-lysosome is compatible with GFP and/or mCherry fluorescent proteins. It can be imaged with standard Cy5 filter sets. SiR-lysosome can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 300 staining experiments*.
λabs 652 nm
λex 674 nm
εmax 1.0·105 mol-1·cm-1
MW 1237.7 g/mol
*Based on the following conditions: 0.3 – 1 ml staining solution / staining experiments with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.
Cytoskeleton, Inc. is the exclusive provider of Spirochrome, Ltd. products in North America.
Live human fibroblast cells stained with 2 µM SiR-lysosome (red) and Hoechst (blue) for 1 h at 37°C and imaged by widefield microscopy and imaged by STED microscopy.
|Caire, Robin et al.||YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitro||Nature Communications 2022 13:1||2022||ISSN 2041--1723|
|Scherer, Katharina M. et al.||A fluorescent reporter system enables spatiotemporal analysis of host cell modification during herpes simplex virus-1 replication||2021||PMID 33380421|
|van der Welle, Reini E N et al.||Neurodegenerative VPS41 variants inhibit HOPS function and mTORC1-dependent TFEB/TFE3 regulation||EMBO molecular medicine||2021||ISSN 1757--4684|
|Özkan, Nazmiye et al.||ER – lysosome contacts at a pre-axonal region regulate axonal lysosome availability||Nature Communications||2021||ISSN 2041-1723|
|Lu, Meng et al.||Sea Cucumber-Derived Peptides Alleviate Oxidative Stress in Neuroblastoma Cells and Improve Survival in C. elegans Exposed to Neurotoxic Paraquat||Oxidative Medicine and Cellular Longevity||2021||ISSN 1942-0994|
|Matthias, Jessica et al.||Cytoplasmic localization of prostate-specific membrane antigen inhibitors may confer advantages for targeted cancer therapies||Cancer Research||2021||ISSN 1538-7445|
|Jongsma, Marlieke LM et al.||SKIP ‐ HOPS recruits TBC 1D15 for a Rab7‐to‐Arl8b identity switch to control late endosome transport||The EMBO Journal||2020||ISSN 0261--4189|
|Valenzuela, José Ignacio et al.||Localized Intercellular Transfer of Ephrin-As by Trans-endocytosis Enables Long-Term Signaling||Developmental Cell||2020||ISSN 1878-1551|