+3
Dbs (Dbl’s big sister) is a guanine nucleotide exchange factor (GEF) that activates the small GTPases Rho and Cdc42, regulating cytoskeletal dynamics, cell adhesion, and migration. It plays key roles in processes such as morphogenesis and oncogenic transformation by promoting actin reorganization and signaling pathway activation.
Human Dbs (DH/PH) protein domain has been produced in a bacterial expression system. It is also His tagged at its amino terminus for purification purposes. The fusion protein has a molecular weight of 40 kDa, it is supplied as a lyophilized protein.
Protein purity is assessed using scanning densitometry of Coomassie-stained SDS-PAGE gels. GE01 is ≥80% pure
The biological activity of GE01 is determined using an in vitro GEF assay. Under the experimental conditions (see datasheet), GE01 increases Cdc42 GTP exchange by ≥5 fold over intrinsic GTPase exchange.
Cat. #GE01-A
