• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIFC3 (kinesin-14B) inhibitors
• Counter-screen for kinesin drug candidates

KIFC3 is a minus-end–directed kinesin motor protein involved in Golgi apparatus positioning and maintenance of cell polarity, particularly in epithelial cells. It plays a critical role in intracellular transport and cytoskeletal organization, contributing to processes such as vesicle trafficking, spindle assembly, cytokinesis, and membrane dynamics.

The wild-type human motor domain of KIFC3 is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag
• Molecular weight: 75 kDa
• Motor amino acids: 384-825
• Uniprot ID: Q9BVG8
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >60% pure.

Biological activity of KIFC3 is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIFC3 exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 80 nmol of ATP produced per minute per mg of KIFC3.