• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF22 inhibitors
• Counter-screen for kinesin drug candidates

KIF22 (also known as Kid/Kinesin-like DNA-binding protein) is a microtubule-based motor protein essential for proper chromosome alignment and segregation during mitosis. Its activity is critical for maintaining genomic stability, and dysregulation has been linked to cell cycle defects and certain cancers.

The wild-type human motor domain of KIF22 is produced in a bacterial expression system.

• Protein tag: N-terminal 6x His tag
• Molecular weight: 43 kDa
• Motor amino acids: 5-378
• Uniprot ID: Q14807
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted to 5 mg/ml, the buffer composition is: 100 mM Tris pH 8.0, 100 mM NaCl, 2 mM MgCl2, 15% (w/v) sucrose, and 1% (w/v) dextran.

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >90% pure.

Biological activity of CS-KF02 is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF22 exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 1200 nmol of ATP produced per minute per mg of KIF22.