• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF7 inhibitors
• Counter-screen for kinesin drug candidates

KIF7 is a human kinesin motor protein that plays a critical role in Hedgehog signaling by regulating the structure and function of the primary cilium. It acts as a key microtubule-associated regulator, essential for proper developmental signaling and tissue patterning.

The wild-type human motor domain of KIF7 is produced in a bacterial expression system.

• Protein tag: N-terminal 6x His tag
• Molecular weight: 44 kDa
• Motor amino acids: 1-370
• Uniprot ID: Q2M1P5.2
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100, 6% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >80% pure.

Biological activity of CS-KF7 is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF7 exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 40 nmol of ATP produced per minute per mg of KIF7.