• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF19 inhibitors
• Counter-screen for kinesin drug candidates

KIF19 is a kinesin motor protein that regulates microtubule dynamics, particularly in cilia, influencing their length and function. It plays an important role in cellular motility and signaling, with potential implications in development and disease.

The wild-type human motor domain of KIF19 is produced in a bacterial expression system.

• Protein tag: N-terminal 6x His tag
• Molecular weight: 41 kDa
• Motor amino acids: 1-353
• Uniprot ID: Q2TAC6
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100, 6% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue stained protein on a4-20% polyacrylamide gel. Purity is determined to be >85% pure.

Biological activity of CS-KF19 is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF19 exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 275 nmol of ATP produced per minute per mg of KIF19.