SiR700-actin is based on the far-red silicon rhodamine (SiR) fluorophore analogue SiR700 and the actin binding natural product jasplakinolide. SiR700-actin allows labeling of endogenous F-actin in live cells with high specificity and low background without the need for genetic manipulation or over-expression. The key features of SiR700-actin are i) far-red absorption and emission wavelengths, ii) cell permeability, iii) fluorogenic character, and iv) compatibility with super-resolution microscopy (e.g., STED & SIM). In addition, SiR700 actin can be used for wide-field and confocal fluorescent imaging in living cells. The emission in the far-red wavelength minimizes phototoxicity and sample autofluorescence. SiR700-actin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filter sets. Probe quantity allows 35 – 140 staining experiments.*
Optical properties
λabs 689 nm
λEm 716 nm
εmax 1.0·105 mol-1·cm-1
MW 1265.6 g/mol
MF C73H88N8O10Si
*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiment with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.
Cytoskeleton, Inc. is the exclusive provider of Spirochrome, Ltd. products in North America.
Swiss 3T3 fibroblasts stained by SiR700-Actin (Cat. # CY-SC013)
For product Datasheets and MSDSs please click on the PDF links below.
Spirochrome Technical Tips and Ex/Em spectra in graphical form (PDF)
