SiR700-Actin Kit

SiR700-Actin Kit
$0.00
SKU
CY-SC013

SiR700-actin is based on the far-red silicon rhodamine (SiR) fluorophore analogue SiR700 and the actin binding natural product jasplakinolide. SiR700-actin allows labeling of endogenous F-actin in live cells with high specificity and low background without the need for genetic manipulation or over-expression. The key features of SiR700-actin are i) far-red absorption and emission wavelengths, ii) cell permeability, iii) fluorogenic character, and iv) compatibility with super-resolution microscopy (e.g., STED & SIM). In addition, SiR700 actin can be used for wide-field and confocal fluorescent imaging in living cells. The emission in the far-red wavelength minimizes phototoxicity and sample autofluorescence. SiR700-actin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filter sets. Probe quantity allows 35 – 140 staining experiments.*

 

Optical properties

λabs   689 nm

λEm   716 nm
εmax 1.0·105 mol-1·cm-1

MW   1265.6 g/mol

MF    C73H88N8O10Si

 

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiment with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

 Cytoskeleton, Inc. is the exclusive provider of Spirochrome, Ltd. products in North America.

3T3_500nM_SiR700-actin_2

Swiss 3T3 fibroblasts stained by SiR700-Actin (Cat. # CY-SC013)

AuthorTitleJournalYearArticle Link
Shou, Jingwen et al.Super-multiplex imaging of cellular dynamics and heterogeneity by integrated stimulated Raman and fluorescence microscopyiScience2021
Kalargyrou, Aikaterini A et al.Nanotube‐like processes facilitate material transfer between photoreceptorsEMBO reports2021
Domart, Florelle et al.Correlating sted and synchrotron xrf nano-imaging unveils cosegregation of metals and cytoskeleton proteins in dendriteseLife2020