SiR700-tubulin is based on the far-red silicon rhodamine (SiR) fluorophore analogue SiR700 and the microtubule-binding drug Docetaxel. SiR700-tubulin allows labeling of endogenous microtubules in live cells with high specificity and low background without the need for genetic manipulation or over-expression. The key features of SiR700-tubulin are i) far-red absorption and emission wavelengths, ii) cell permeability, iii) fluorogenic character, and iv) compatibility with super-resolution microscopy (e.g., STED & SIM). In addition, SiR700-tubulin can be used for wide-field and confocal fluorescent imaging in living cells. The emission in the far-red wavelength minimizes phototoxicity and sample autofluorescence. SiR700-tubulin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filter sets. Probe quantity allows 35 – 140 staining experiments.*
λabs 689 nm
λEm 716 nm
εmax 1.0·105 mol-1·cm-1
MW 1327.6 g/mol
*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiment with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration
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Spirochrome Technical Tips and Ex/Em spectra in graphical form (PDF)
|Tsuchiya, Kenta et al.||Ran-GTP Is Non-essential to Activate NuMA for Mitotic Spindle-Pole Focusing but Dynamically Polarizes HURP Near Chromosomes||Current Biology||2021||ISSN 1879-0445|