• Fluorogenic, far-red F-actin labeling probe, extremely low background for live cell actin imaging
• Fluorogenic stain for actin filaments in paraformaldehyde fixed cells

SiR-actin is based on the fluorophore silicon rhodamine (SiR) and the actin-binding natural product jasplakinolide. SiR-actin allows the labelling of F-actin in live cells with high specificity and low background. Its superior fluorogenic properties make this the probe of choice for low-background imaging of dynamic actin filaments. FastAct™ /FastAct™_X probes are recommended for imaging highly dynamic actin processes.

Key features

• Ab: 652 nm, Em: 674 nm – far-red spectrum
• Emission in far-red minimizes phototoxicity and sample autofluorescence
• Cell-permeable
• Most suited to imaging actin filaments rather than fast actin dynamics
• Works on live or fixed cells
• Fluorogenic character: enhanced fluorescence when bound to actin filaments. Most fluorogenic of all the live cell actin probes
• Compatible with super-resolution microscopy (STED & SIM)

The biological activity of CY-SC001 is assessed by the ability of the probe to efficiently label actin filaments in live cell culture. After a wash step, cell staining is visible for several hours.