MemGlow NR4A is a solvatochromic photostable plasma membrane-targeting dye with on/off switching that has been optimized for Super Resolution Point Accumulation for Imaging in Nanoscale Topography (SR-PAINT). Upon binding with a plasma membrane in a predominant liquid ordered (LO) phase NR4A exhibits a 45-50 nm wavelength shift relative to liquid disordered (Ld) phase enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma membranes.
NR4A Membrane Polarity Probe Product Functions (MG06)
For more detailed information on using this product see the About Tab
** 2 nmol of MemGlow will produce a 20µM stock solution when reconstituted with 100 µl of anhydrous DMSO
Figure 3. A) Super resolution imaging of live HeLa cells labeled with 10 nM NR4A (false colored red). B) Widefield fluorescent imaging of live HeLa S1 cells labeled with 10 nM NR4A. HeLa S1 cells were imaged with a TRITC filter set, a digital CCD camera, and 100x oil objective (false colored red).
Figure 1. Diagram of NR4A’s lipid order-dependent emission.
Figure 2. Absorbance and excitation spectra of MemGlow NR4A diluted in DMSO to 4.8 µM. Absorbance peak detected at 553 nm with peak emission at 637 nm.
Figure 3. Fluorescence spectra of NR4A in Lo (Cholesterol) and Ld (DOPC) phases.
The absorption max of NR4A is 554 nm, with an emission spectra of 638 nm, an extinction coefficient of 45,000 M-1.cm-1 , and can be visualized using a Cy 3.5 filter set or other suitable filter sets. NR4A is supplied as a lyophilized pellet. NR4A is a lipid binding dye and appropriate PPE should be worn at all times. Dispose of NR4A according to local regulations and policies.
Storage and Reconstitution
The lyophilized product is stable at 4°C (<10% humidity) for 6 months and should be protected from light. To reconstitute, briefly centrifuge to collect the product at the bottom of the tube. NR4A should be reconstituted with 100 µl of anhydrous DMSO to create a 20 µM stock solution for cell imaging. After reconstitution the solution should be stored at -20°C where it is stable for 3 months. Once reconstituted, allow product to warm to room temperature before opening tube.
Important Technical Notes
Note. Optimal conditions for efficient labeling should be determined for each cell line and application.
MEMBRIGHT™ is a trademark of CNRS/UNISTRA of France.
|Danylchuk, Dmytro I. et al.||Switchable Solvatochromic Probes for Live-Cell Super-resolution Imaging of Plasma Membrane Organization||Angewandte Chemie International Edition||2019||ISSN 1521--3773|
|Kucherak, Oleksandr A. et al.||Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes||Journal of the American Chemical Society||2010||ISSN 0002-7863|