• Fluorogenic plasma membrane probe
• Detect changes in plasma membrane polarity
• Label extracellular vesicles

MemGlow 400™Pro12A is an analogue of the commonly used dye Laurdan, which was designed for specific labelling of the cell plasma membrane outer leaflet. Owing to its solvatochromism, Pro12A can also be used to visualize lipid organization of cell membranes. Upon binding with a plasma membrane in a predominant liquid ordered (LO) phase, Pro12A exhibits a 45-50 nm wavelength shift relative to the liquid disordered (Ld) phase, enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma

Key features

• Fluorogenic probe: Ab: 363 nm, Em: 506 nm – green – spectrum
• Polarity shift: 499 ±23 nm (ordered), <446 ±23 nm (disordered)
• Engineered for confocal and two-photon excitation techniques, also compatible with widefield

The biological activity of MG09 is assessed by the ability of the probe to efficiently label plasma membranes in live HEK293 cell culture.