• Study in vitro acetylation of α-tubulin
• Study biochemistry of alpha tubulin N-acetyl transferase 1 (αTAT1)

Acetylation of lysine 40 (K40) on the luminal side of α-tubulin is the most studied tubulin modification, catalyzed specifically by the transferase αTAT1. Abnormal K40 acetylation has been linked to neurodegenerative diseases, ciliopathies, and various cancers.
Human αTAT1 protein (aa 2-236) is produced in a bacterial expression system. The recombinant protein is untagged and has a molecular weight of 27 kDa. The protein is supplied as a lyophilized powder.

Protein purity is assessed by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gel. Purity is determined to be ≥90%.

The biological activity of αTAT1 is assessed by its ability to acetylate tubulin as demonstrated via Western blot analysis. See datasheet for details.