+3
Fibronectin is purified from bovine plasma; it is a high-molecular-weight (~440kDa) glycoprotein, made up of two subunits that vary in size between 235-270 kDa (due to alternate splicing). The secreted fibronectin dimer is a soluble protein that polymerizes to higher-order fibrils in the extracellular matrix (ECM).
The protein is modified to contain covalently linked long-chain biotins at random surface lysines; a long-chain is used to avoid steric hindrance in downstream applications.
Protein purity is determined by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. FNR03 is >80% pure.
Biological activity of FNR03 is determined by the ability of the soluble reagent to incorporate into extracellular fibrillar matrices. This assay is a non-radioactive alternative to the quantification of matrix assembly in response to various signaling events.
Lot-to-lot labeling efficiency is assessed by the ability to detect 10 ng biotinylated fibronectin using alkaline phosphatase-conjugated streptavidin.
Cat. #FNR03
