• Study in vitro regulation of K-Ras4B G12D cell signaling mechanisms
• Identification of K-Ras4B G12D exchange factors (GEFs)

K-Ras is a small GTP-binding protein that acts as a molecular switch, regulating cell growth, differentiation, and survival through key signaling pathways. Mutations in K-Ras are common in various cancers, leading to uncontrolled cell proliferation and tumor development.

G12D is recognized as one of the most frequent KRAS mutants, commonly found in pancreatic cancers.

Human K-Ras4B G12D protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: mwt 23-25 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 5 mg/ml, the buffer composition is: 50 mM Tris, pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥85% pure.

The biological activity of K-Ras4B G12D is determined from the ability of the SOS1 exchange domain (SOS1-ExD) to catalyze the exchange of GDP for GTP onto mutant K-Ras4B.