• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF18B inhibitors
• Counter-screen for kinesin drug candidates

KIF18B is a kinesin motor protein involved in regulating microtubule dynamics during mitosis, ensuring accurate chromosome alignment and segregation. It plays a critical role in spindle function and cell division, making it essential for maintaining genomic stability.

The wild-type human motor domain of KIF18B is produced in a bacterial expression system.

• Protein tag: N-terminal 6x His tag
• Molecular weight: 44 kDa
• Motor amino acids: 1-374
• Uniprot ID: Q86Y91
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted to 5 mg/ml, the buffer composition is: 100 mM Tris pH 8.0, 100 mM NaCl, 2 mM MgCl2, 15% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >80% pure.

Biological activity of CS-KF18B is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF18B exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 1500 nmol of ATP produced per minute per mg of KIF18B.