Advance your actin research—unlabeled or pyrene-labeled oxidized actin lets you analyze methionine oxidation’s impact on actin dynamics. High purity, trusted performance, and ready for discovery.

• Study the role of M44/47 oxidation on actin function
• Compare the in vitro functionality of oxidized vs non-oxidized actin in your experimental system

Regulation of actin oxidation at Met44/Met47 has been shown to destabilize F-actin in vivo and to play a key role in a growing number of cellular processes, including cytokinesis, axonal guidance, dendritic organization, synaptic development, heart and muscle development, and cell viability.

Rabbit skeletal muscle actin protein AKL99:AKL99 is enzymatically oxidized at methionine 44 and 47 (-actin nomenclature) with the MICAL flavoprotein monooxygenase protein Cat. # MC01.

• Molecular weight ~43 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 1 mg/ml, the buffer composition is: 5 mM Tris-HCl, pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5% (w/v) sucrose, and 1% (w/v) dextran.

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity is determined to be ≥99% pure.

A subtilisin limited proteolysis assay determines that actin is ≥90% oxidized at M44/47 (see datasheet for details). An in vitro polymerization pelleting assay determines that the oxidized actin is able to form actin filaments (>90% polymer).