Rac proteins are small GTP-binding proteins that regulate actin-based structures such as lamellipodia, membrane ruffles, and dorsal ruffles, enabling dynamic remodeling of the cytoskeleton. They are essential for processes including cell migration, adhesion, and growth factor–mediated signaling.
The constitutively active form of human Rac1 protein is produced in a bacterial expression system. The protein has a glutamine to leucine substitution at amino acid 61, creating a constitutively active mutant protein that will not hydrolyze GTP.
Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.
The biological activity of R6101 is measured by its ability to mimic a GTPγS-loaded wild-type Rac1 in a G-LISA assay BK128.
Cat. #R6101
