• Visualize fluorescent fibronectin incorporation, remodeling, and degradation in live-cell imaging studies of cell/ECM interactions
• FACS analysis of fibronectin-binding cells
• Cell invasion assays
• Mechanobiology and tissue engineering research

Fibronectin is purified from bovine plasma; it is a high-molecular-weight (~440kDa) glycoprotein, made up of two subunits that vary in size between 235-270 kDa (due to alternate splicing). The secreted fibronectin dimer is a soluble protein that polymerizes to higher-order fibrils in the extracellular matrix (ECM).

The protein is modified to contain covalently linked rhodamines at random surface lysines.

• Ab: 535nm Em: 585 nm – orange/red spectrum
• Labeling stoichiometry: ~1-3 dyes per fibronectin dimer
• <5% free dye detectable in the final product
• Supplied as a lyophilized powder

Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gel. FNR01 is >80% pure.

Biological activity of FNR01 is demonstrated by its ability to act as a substrate for cell invasion as monitored through an ECM degradation assay.