• Drug discovery reagent for Ras/SOS2 GEF inhibitors
• Characterize GEF functions of SOS2

Son of Sevenless 2 (SOS2) is a guanine nucleotide exchange factor (GEF) that activates Ras proteins by facilitating the exchange of GDP for GTP, thereby triggering Ras-dependent signaling pathways.
The SOS2 exchange domain (amino acids 563-1051) of the human SOS2 protein has been produced in a bacterial expression system. It is also 6xHis tagged at its amino terminus for purification purposes. The fusion protein has a molecular weight of 61 kDa, and it is supplied as a lyophilized protein.

Protein purity is assessed using scanning densitometry of Coomassie-stained SDS-PAGE gels. CS-GE08 is ≥90% pure

The biological activity of CS-GE08 is determined using an in vitro GEF assay. Under the experimental conditions (see datasheet), CS-GE08 increases K-Ras4B GTP exchange by ≥5 fold over intrinsic K-Ras4B exchange.