• Fluorogenic, yellow/orange stain for DNA in live cells
• Fluorogenic yellow/orange stain for DNA in fixed cells and tissues
• Compatible with multicolor imaging

SPY555-DNA reagent is a conjugate of SPY™555 dye and the DNA minor groove binder bisbenzimide (Hoechst). It allows the labelling of DNA in live cells with high specificity and low background.

Key features

• Ab: 555 nm, Em: 580 nm – yellow/orange spectrum
• Cell-permeable
• Bright, non-toxic
• Works on live cells and on fixed cells or tissues
• Fluorogenic character: enhanced fluorescence when bound to DNA
• Compatible with widefield, confocal, SIM, or STED imaging

The biological activity of CY-SC201 is assessed by the ability of the probe to label DNA in live HeLa cell culture efficiently. After an optional wash step, cell staining is visible for several hours. Omit the wash step for time-lapse imaging.