• Fluorogenic, orange stain for DNA in live cells and tissues
• Fluorogenic, orange stain for DNA in fixed cells and tissues
• Fluorogenic, orange nuclear stain for live and fixed cells and tissue

SPY™595-DNA is based on the SPY™ dye conjugated to the DNA minor groove binder bisbenzimide (Hoechst). It allows the labelling of DNA in live and fixed cells and tissues with high specificity and low background.

Key features

• Ab: 599 nm, Em: 615 nm – orange spectrum
• Cell-permeable
• Bright, non-toxic
• Works in live and fixed cells or tissues
• Fluorogenic character: enhanced fluorescence when bound to DNA
• Compatible with widefield, confocal, SIM, or STED imaging

The biological activity of CY-SC301 is assessed by the ability of the probe to efficiently label DNA in live HeLa cell culture. After an optional wash step, cell staining is visible for several hours. Omit the wash step for time lapse imaging.