SPY700-tubulin

• Fluorogenic far-red probe for imaging microtubules in live cells and tissues
• Supports multicolor imaging

SPY700-tubulin is a high-performance, cell-permeable fluorescent probe designed for selective visualization of microtubules in living cells. By combining the microtubule-binding properties of docetaxel with the bright, far-red SPY fluorophore, SPY700-tubulin enables high-contrast imaging of microtubule networks with exceptional specificity and minimal background fluorescence. Its cell-permeable design allows direct labeling of endogenous microtubules without the need for genetic manipulation, providing a convenient and powerful tool for investigating microtubule organization and dynamics in live cells.

Key features

• Ab: 696 nm, Em: 718 nm-far red spectrum
• Emission in far-red minimizes phototoxicity and sample autofluorescence
• Cell permeable
• Bright
• Fluorogenic character: enhanced fluorescence when bound to microtubules
• Compatible with widefield, confocal, STED & SIM imaging
• Fluorescence lifetime: 2.4 ns
• STED depletion wavelength: 780-820 nm

The biological activity of CY-SC603 is assessed by the ability of the probe to efficiently label microtubules in live cell HeLa culture. After an optional wash step, cell staining is visible for several hours.
SPY700-tubulin is a fluorescent taxane-based probe that binds specifically to microtubules. As high concentrations of taxane derivatives may affect microtubule dynamics, care should be taken to minimize potential effects on normal cellular function. For long-term live-cell imaging (>12 h), a dilution of at least 1:1,000 from the probe stock solution is recommended. Optimal staining concentrations should be determined empirically for each cell type and experimental application.