• Validated for Western blot-WB

Anti-ubiquitin antibody (AUB01) was raised against full-length bovine ubiquitin protein. It detects a broad profile of ubiquitinated proteins and free ubiquitin in cell lysates. AUB01 is purified by Protein G affinity chromatography. The clone is licensed from Fred Hutchinson Cancer Research Center; the antibody is made in-house by Cytoskeleton.

Host/isotype/clone:

• Mouse/IgG1
• Clone: P4D1

Reactivity:

• Broad cross-species reactivity

Amount of material:

• AUB01: 2 x 100 µl when reconstituted (110-130 µg per tube)
• AUB01-S: 1 x 25 µl when reconstituted (27.5-32.5 µg per tube)
• AUB01-XL: 4 x 500 µl when reconstituted (550-650 µg per tube)

Working concentrations:

• WB 1:500 dilution 2.2-2.5 µg/ml final 100 ml working volume - full size Ab

Formulation

• Supplied as a lyophilized powder
• Buffer composition after reconstitution in 50% glycerol in water: 50 mM PIPES pH7.0, 1% sucrose, 0.5% dextran, 50% glycerol

Sensitivity:

• Detects ≤3 ng bovine thymus ubiquitin protein

Specificity:

• In 20 µg of Swiss 3T3 cell lysates, a broad range of ubiquitinated proteins and free ubiquitin are detectable. Treatment with 10 µM MG132 for 5 h increases ubiquitinated species by 2–3 fold