+3
Tiam1 is a guanine nucleotide exchange factor (GEF) that activates Rho family GTPases by catalyzing the exchange of GDP for GTP, thereby regulating actin cytoskeleton dynamics and cell signaling. Its GEF activity plays a key role in processes such as cell migration, adhesion, and morphological changes.
The DH/PH domain of the human Tiam1 protein (amino acids 1040–1406) has been produced from a bacterial system, and it is fused at its N-terminus to a maltose-binding protein (MBP). The resulting MBP–Tiam1-DH/PH fusion protein has a molecular weight of approximately 86 kDa and is provided as a lyophilized powder.
Protein purity is assessed using scanning densitometry of Coomassie-stained SDS-PAGE gels. CS-GE04 is ≥90% pure
The biological activity of CS-GE04 is determined using an in vitro GEF assay. Under the experimental conditions (see datasheet), CS-GE04 increases Rac1 GTP exchange by ≥5 fold over intrinsic Rac exchange.
Cat. #CS-GE04
