• Study in vitro regulation of Cdc42 cell signaling mechanisms
• Serves as a substrate for Cdc42 GEF assays and drug screens
• Serves as a substrate for Cdc42 GAP assays and drug screens

Cdc42 is a small GTP-binding protein in the Rho family that regulates cell polarity, cytoskeletal organization, and signal transduction. It is best known for controlling the formation of filopodia—thin, actin-rich projections that help cells sense their environment and guide directional movement.

Wild-type human Cdc42 protein is produced in a bacterial expression system.

• Protein tag: N-terminal His tag for purification
• Molecular weight: approximate mwt 22-25 kDa
• Formulation: supplied as a lyophilized powder
• Composition: when resuspended to 1 mg/ml, the buffer composition is: 10 mM Tris, pH 7.5, 10 mM NaCl, 0.1 mM MgCl2, 1.0% sucrose, and 0.2% dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. Purity was determined to be ≥90% pure.

The biological activity of CD01 is measured by its ability to exchange GTP. A standard assay uses GST-tagged PAK-1 PBD beads Cat. # PAK02 to detect GTP-bound Cdc42-His preloaded with GTPγS or GDP. The PAK CRIB domain specifically binds the active (GTP-bound) form of Cdc42. Quality control ensures ≥70% binding with GTPγS-loaded Cdc42 and ≤10% binding with GDP-loaded Cdc42.