+3
Vav2 is a guanine nucleotide exchange factor (GEF) that activates members of the Rho family of small GTPases, particularly Rac1, by catalyzing the exchange of GDP for GTP. It plays a central role in linking extracellular signals to changes in the actin cytoskeleton, cell migration, and intracellular signaling cascades.
The DH domain of human Vav2 protein (amino acids 189-374) has been produced in a bacterial expression system. It is also 6xHis tagged at its amino terminus for purification purposes. The fusion protein has a molecular weight of 25 kDa, it is supplied as a lyophilized protein.
Protein purity is assessed using scanning densitometry of Coomassie-stained SDS-PAGE gels. CS-GE06 is ≥80% pure
The biological activity of CS-GE06 is determined using an in vitro GEF assay. Under the experimental conditions (see datasheet), CS-GE06 increases Rac1 GTP exchange by ≥5 fold over intrinsic Rac1 exchange.
Cat. #CS-GE06
