Anti-Cdc42: mouse Mab

Anti-Cdc42: mouse Mab
$0.00

Introduction
Cdc42 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Cdc42 is expressed in a large number of different cell types.

 

Product Uses Include
This antibody is recommended for detection of Cdc42 in human, mouse, rat or other extracts.  The following protocols have been tested with this antibody: 

 

Western BlotImmunocytochemistryELISAImmunoprecipitation
YesYesNoNo

 

RRID

AB_10716593

Material
The anti-Cdc42 antibody (Cat. # ACD03) is a mouse monoclonal antibody that only recognizes Cdc42, not Rac1, Rac2, Rac3, RhoA, RhoB, RhoC or H-Ras. Other commercially available Cdc42 antibodies recognize one or more of these close isoforms  Platelet extract (Cat. # EXT01) is included as a positive control. ACD03 is supplied as a lyophilized white powder.

 

ACD03west-01

Figure 1: Western blot analysis of purified protein and cell extracts using ACD03 as primary antibody. Recombinant small G-proteins and tissue extracts were separated by SDS-PAGE and transferred to PVDF membrane.  ACD03 was diluted to 250 ng/ml (1:1000) in PBST plus 0.1% non-fat milk powder and Western analysis was as described in the Methods section of the datasheet. Lane 1; 25 ng Cdc42-6xHIS, Lane 2; 250 ng RhoA-6xHIS, Lane 3; 250 ng Rac1-6xHIS, Lane 4; 40 µg Swiss 3T3 cell extract, Lane 5; 40 µg bovine brain extract, Lane 6; 40 µg human platelet extract. Note: Cdc42-6xHIS runs at 25kDal whereas the native Cdc42 runs at 22kDal.

Table 1 - Reactivity of other commercially available Cdc42 antibodies

Cdc42

Rac sub-family

Rho sub-family

Ras sub-family

Cytoskeleton, Inc. (Cat. # ACD03)

Yes

No

No

No

Santa Cruz (Cat. #sc-87-G)

Yes

Yes

Yes

NT

Cell Signalling (Cat. # 2462)

Yes

Yes

NT

NT

NT = Not tested

For product Datasheets and MSDSs please click on the PDF links below.

Sample Size Datasheet (Cat. ACD03B-S):  

AuthorTitleJournalYearArticle Link
Kamata, Tamihiro et al.Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironmentDMM Disease Models and Mechanisms2022ISSN 1754-8411
Barbera, Stefano et al.The C-type lectin CD93 controls endothelial cell migration via activation of the Rho family of small GTPasesMatrix Biology2021ISSN 1569-1802
Howden, Jake D. et al.α2β1 integrins spatially restrict Cdc42 activity to stabilise adherens junctionsBMC biology2021ISSN 1741-7007
Li, Jingxuan et al.Eva1a ameliorates atherosclerosis by promoting re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1bCardiovascular Research2021ISSN 1755-3245
Jayabal, Panneerselvam et al.NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growthCell Reports2021ISSN 2211-1247
Hosseini, Kamran et al.EMT-Induced Cell-Mechanical Changes Enhance Mitotic Rounding StrengthAdvanced Science2020ISSN 2198-3844
Gorisse, Laetitia et al.Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42Journal of Biological Chemistry2020ISSN 1083-351X
Gu, Jiawen et al.Rho-GEF trio regulates osteoclast differentiation and function by Rac1/Cdc42Experimental Cell Research2020ISSN 1090-2422
Oni, Tobiloba E. et al.SOAT1 promotes mevalonate pathway dependency in pancreatic cancerJournal of Experimental Medicine2020ISSN 1540-9538
Vestre, Katharina et al.Rab6 regulates cell migration and invasion by recruiting Cdc42 and modulating its activityCellular and Molecular Life Sciences2019ISSN 1420-9071
Singh, Rajesh K. et al.Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and CatabolismArteriosclerosis, Thrombosis, and Vascular Biology2019ISSN 1524-4636
Dupraz, Sebastian et al.RhoA Controls Axon Extension Independent of Specification in the Developing BrainCurrent Biology2019ISSN 0960-9822
Huang, Yuxing et al.Arp2/3-branched actin maintains an active pool of GTP-RhoA and controls RhoA abundanceCells2019ISSN 2073-4409
MacKeil, Jodi L. et al.Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cellsCellular Signalling2019ISSN 1873-3913
Li, Cao et al.Regulation of staphylococcus aureus infection of macrophages by CD44, reactive oxygen species, and acid sphingomyelinaseAntioxidants and Redox Signaling2018ISSN 1557-7716
Stypulkowski, Ewa et al.The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell divisionScience Signaling2018ISSN 1937-9145
Kawasaki, Natsumi et al.TUFT1 interacts with RABGAP1 and regulates mTORC1 signalingCell Discovery2018ISSN 2056-5968
Ghézali, Grégory et al.Connexin 30 controls astroglial polarization during postnatal brain developmentDevelopment (Cambridge)2018ISSN 1477-9129
Vodicska, Barbara et al.MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progressionScientific Reports2018ISSN 2045-2322
Wu, Nan et al.RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activationBMC Cancer2018ISSN 1471-2407
Lam, Jonathan G.T. et al.Host cell perforation by listeriolysin O (LLO) activates a Ca2+-dependent cPKC/Rac1/Arp2/3 signaling pathway that promotes Listeria monocytogenes internalization independently of membrane resealingMolecular Biology of the Cell2018ISSN 1939-4586
Liu, Xiaolei et al.Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctionsDevelopment (Cambridge)2018ISSN 1477-9129
Peretti, Amanda S. et al.The R-Enantiomer of Ketorolac Delays Mammary Tumor Development in Mouse Mammary Tumor Virus-Polyoma Middle T Antigen (MMTV-PyMT) MiceAmerican Journal of Pathology2018ISSN 1525-2191
Dudvarski Stanković, Nevenka et al. EGFL7 enhances surface expression of integrin α 5 β 1 to promote angiogenesis in malignant brain tumors EMBO Molecular Medicine2018ISSN 1757--4676
Saito, Masaki et al.Tctex‐1 controls ciliary resorption by regulating branched actin polymerization and endocytosisEMBO reports2017ISSN 1469--221X
Bucka, Kenneth B. et al.Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturationMolecular Biology of the Cell2017ISSN 1939-4586
Chang, Ting Ya et al.Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machineryeLife2017ISSN 2050-084X
Takeuchi, Hiroki et al.Intracellular periodontal pathogen exploits recycling pathway to exit from infected cellsCellular Microbiology2016ISSN 1462-5822
Seidelin, Jakob Benedict et al.Cellular inhibitor of apoptosis protein 2 controls human colonic epithelial restitution, migration, and Rac1 activationAmerican Journal of Physiology - Gastrointestinal and Liver Physiology2015ISSN 1522-1547
Thomas, Audrey et al.Involvement of the Rac1-IRSp53-Wave2-Arp2/3 Signaling Pathway in HIV-1 Gag Particle Release in CD4 T CellsJournal of Virology2015ISSN 0022--538X
Liu, Chunqiao et al.Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5aBiology Open2014ISSN 2046-6390
David, Muriel D. et al.The RhoGAP ARHGAP19 controls cytokinesis and chromosome segregation in T lymphocytesJournal of Cell Science2014ISSN 0021-9533
Valtcheva, Nadejda et al.The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42Journal of Biological Chemistry2013ISSN 0021-9258
Barrio, Laura et al.TLR4 Signaling Shapes B Cell Dynamics via MyD88-Dependent Pathways and Rac GTPasesThe Journal of Immunology2013ISSN 0022--1767
Steele, Brian M. et al.WNT-3a modulates platelet function by regulating small GTPase activityFEBS Letters2012ISSN 0014-5793
Mercer, Jason et al.Vaccinia virus strains use distinct forms of macropinocytosis for host-cell entryProceedings of the National Academy of Sciences of the United States of America2010ISSN 0027-8424

 

Question 1: What is the antigen that this antibody was raised against?

Answer 1: The antibody has been raised against a peptide sequence of human Cdc42 (amino acids 128-138).

 

Question 2:  What species has this antibody been tested against?

Answer 2:  This antibody is recommended for western blot detection of Cdc42 in human, mouse, rat and bovine cell/tissue extracts.

 

 

 If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com